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World Journal of Pharmaceutical
and Medical Research

An International Peer Reviewed Journal for Pharmaceutical and Medical Research and Technology
An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)
ISSN 2455-3301
IMPACT FACTOR: 4.639

ICV : 78.6

Abstract

SIGNAL AMPLIFICATION OF SIA-CHEMILUMINESCENCE PROBE FOR METHOTREXATE DETECTION IN PHARMACEUTICAL FORMULATIONS USING SILVER NANOPARTICLES

Nabeel Alsaffar, Abdulkareem Abdulraheem, Maha F. El-Tohamy* and Hadi Abbas Alnajjar

ABSTRACT

This work focused on the development of a sequential injection chemiluminescence (SIA-CL) system luminolferricyanide (III) in the presence of silver nanoparticles (AgNPs) for the detection of methotrexate (MTX) in its pharmaceutical formulations. The experimental conditions were optimized and the CL detection was conducted by measuring the high catalytic potential of AgNPs on the CL oxidation reaction. It was found that the increase in CL signal is proportional to the concentration of the target analyte. The calibration graph of the SIA-CL system was constructed covering a linear concentration range of 0.01-100 ?g mL-1 for MTX in the presence of AgNPs. The influence of possible interfering species such as common cations, amino acids, sugars and coformulated additives was tested. The suggested method was validated in accordance with ICH guidelines. The suggested SIA-CL system was successfully applied to determine the investigated drug in bulk powder and pharmaceutical dosage forms. The overcome results were in good agreement with those obtained by other published method.

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