IMMUNOHISTOCHEMICAL CHARACTERIZATION OF PRODUCT OF CONCEPTION USING P53 AND Ki67 AMONG WOMEN IN BAYELSA STATE SOUTH-SOUTH NIGERIA
*Eric E. U., Akele R. Y., Echeonwu B. C. and Odumah E N.
Background; Products of conception, abbreviated poc, is medical term used for tissue derived from the union of an egg and a sperm. It encompasses an embryonic gestation (blight ovum) which does not have a viable embryo. The prognostic indicators based on currently available clinical and histopathological variables such as villous size, outlines, stroma and hydrops etc. However, these indicators are still inadequate. Objectives; To characterize the various moles (complete and incomplete moles) using various genes expressed and to determine the extend of percentage expression of the individual genes. Materials and Method; A total of fifty (50) slides of product conception tissues were collected of different age’s and ethnicity, the histological characterization and morphological evaluation were defined using the routine (Haematoxglin and Eosin) staining method into 20 complete moles and 30 incomplete moles. Immunostaining was done for P53 and ki67 using the manufacturer’s antibodies. Result; The result are presented in photomicrograph and tables. the expression of p53 gene in product of conception. A total of 50 cases are used for the study 20 (66.7%) complete moles with 9 (18.0%) positive expression and 11 (22.0%) negative expression. 30 (60.0%) incomplete moles with 5 (10.0%) positive and 25 (50.0%) negative expression the expression of ki67 gene in product of conception 20 (66.7%) complete moles with 0 (0.0%) positive and 20 (66.7%) negative expression,30 (60.0%) incomplete moles with 0 (0.0%) positive expression and 30 (60%) negative expression. Conclusion; This study shows that based on the percentage of expression, p53 gene has a good sensitivity and with good prognosis as oppose to ki67. Therefore P53 immunohistochemical stain should be incorporated together with haematoxylin and eosin for differentiation of complete and incomplete moles.[Full Text Article]