EVALUATİON OF ANTİ-BİOFİLM AND ANTİFUNGAL ACTİVİTİES OF MENTHA LONGİFOLİA ESSENTİAL OİL AGAİNST CLİNİCAL ISOLATES OF CANDİDA ALBİCANS
Uğur Tutar*
ABSTRACT
Mentha longifolia is traditionally used in the treatment of various diseases such as digestive and headache disorders. In current study, the essential oil of M. longifolia (EOML) was investigated for their antimicrobial, antibiofilm activity and chemical composition. The EOML chemical composition was analyzed by gas chromatography-mass spectrometry. Antimicrobial and anti-biofilm properties were determined using broth microdilution methods. According to the results of this study, the GC-MS profile highlights that the mains compounds were determined (61.40 %) Carvacrol, (17.38 %) o-Cymol, and (8.27 %) ?-Terpinene. The antifungal activity of the EOML was evaluated by the micro-dilution method on Candida albicans clinical isolates. It was determined that EOML inhibited 10 tested yeasts with Minimum Inhibitory Concentration (MIC) values in the range of 1.56- 3.12 ?l mL-1. Minimum Biofilm Inhibitory Concentration (MBIC) value was found to be range of 3.12-12.5 (?l mL-1) while the Minimum Biofilm Eradication Concentration (MBEC) value was found to be 3.12- 6.25 (?l mL-1). MIC value of the M. longifolia essential oil was applied onto the C. albicans biofilm formations. It was seen that the EOML reduced the cell viability in biofilm by 20.3-71.0 %. The findings of this study show that EOML has antifungal and anti-biofilm activity against C. albicans isolates. For this reason, M. longifolia essential oil may be considered as a potential agent for the treatment of C. albicans infections associated with biofilm.
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