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World Journal of Pharmaceutical
and Medical Research

An International Peer Reviewed Journal for Pharmaceutical and Medical Research and Technology
An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)
ISSN 2455-3301
IMPACT FACTOR: 4.639

ICV : 78.6

Abstract

A MODIFIED REVERSE PHASE LIQUID CHROMATOGRAPHIC METHOD DEVELOPMENT AND VALIDATION FOR THE SIMULTANEOUS ESTIMATION OF GLIMEPRIDE AND PIOGLITAZONE IN BULK AND DOSAGE FORMS

Kavuri Dwarathi*, D. Dhachinamoorthi

ABSTRACT

A modified reverse phase liquid chromatographic method was developed and validated for the simultaneous estimation of glimepride and Pioglitazone in bulk and pharmaceutical dosage forms. The developed and validated method was simple, precise, accurate and economical reverse phase liquid chromatography method. Estimation of drugs in this combination was done with a C18 column (Zorbax 100-5 column, 250mm x 4.6mm) passing mobile phase of composition phosphate buffer pH 7: Acetonitrile in ratio 45:55 v/v. The flow rate was 1 ml/min and the column effluents were identified at 230 nm. The retention time of Pioglitazone and Glimepiride were 6.7 min and 8.3 min respectively. The method was found to be linear over a range of 10-50?g/ml for Pioglitazone and 3-15?g/ml for Glimepiride. The validated method proved as reproducible one with a %RSD value of less than 2 and having the selectivity and accuracy within the specified limits. Assay of marketed dosage forms was determined and found to be with 98% and 99% for Pioglitazone and Glimepiride respectively. The method was validated according to the guidelines of International Council for Harmonization (ICH) and was successfully utilized in the simultaneous estimation of the commercial dosage forms. This liquid chromatographic method can be applied for the qualitative and quantitative determination of selected drugs.

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