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World Journal of Pharmaceutical
and Medical Research

An International Peer Reviewed Journal for Pharmaceutical and Medical Research and Technology
An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)
ISSN 2455-3301
IMPACT FACTOR: 4.639

ICV : 78.6

Abstract

A MODIFIED REVERSE PHASE LIQUID CHROMATOGRAPHIC METHOD DEVELOPMENT AND VALIDATION FOR THE FLUOXETINE IN BULK AND DOSAGE FORMS

Ramavathu Ravi Naik*, Ch. M. M. Prasada Rao and D. Dhachinamoorthi

ABSTRACT

A modified reverse phase liquid chromatographic method was developed and validated for the A new gradient reverse Phase High Performance Liquid chromatographic method development for the determination of Fluoxetine HCL tablet dosage form. The chromatographic separation was achieved on a Thermo Zorbax SB C8, 150×4.6mm,5?, column within a runtime of 2.06 min under gradient elution by a mixture of buffer and acetonitrile and water at a flow rate of 0.8ml/min. A photodiode array (PDA) detector set at 216 nm was used for detection. The spectrophtometric method was developed for the Zofirlukast at uv cut of range of 200-400nm here the absorption max was shown at 216 nm. The method was validated according to the ICH guidelines with respect to specificity, precision, accuracy and linearity, and showing satisfactory data for all the method validation parameters tested. The % RSD for peak area response was found to be within the limit. The proposed method was found to be reproducible and convenient for the analysis of Fluoxetine HCL, the method can be used for routine quality control analysis.

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