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World Journal of Pharmaceutical
and Medical Research

An International Peer Reviewed Journal for Pharmaceutical and Medical Research and Technology
An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)
ISSN 2455-3301
IMPACT FACTOR: 4.639

ICV : 78.6

Abstract

WATER QUALITY OF SURFACE WATER BODIES AND ANTIMICROBIAL SUSCEPTIBILITY PATTERN OF PSEUDOMONAS SPECIES IN RIVERS STATE, NIGERIA

Wilcox Inyingierfagha Moses, Obire Omokaro and Wemedo Samuel Amadi

ABSTRACT

The study was aimed at determining the antimicrobial susceptibility pattern of Pseudomonas species associated
with surface water bodies in three selected rivers (Bonny, Mgboshimini and Ogbogoro) in Port Harcourt
Metropolis, Rivers State. Thirty-six samples were collected from the rivers designated stations A, B, and C
respectively amid two seasons (Dry and raining) and analyzed for physico-chemical and microbiological
characteristics using standard analytical procedures. Result obtained from physico-chemical analysis varied within
the two seasons, the pH of the samples from the three different Stations during dry season ranged from 7.10 -7.20
while that of rainy season was from 7.20 -9.10. Total suspended solids during dry season ranged from 32.70 –
53.50mg/L showing significant variation from WHO Standard which is 30mg/L, total dissolved solids for rainy
season ranged from 640 -2800mg/L while Station A had the highest value of 2110mg/L for Total dissolved solids
during dry season these were all significantly high in comparison with WHO standard. The mean total
heterotrophic bacterial counts (CFU/ml) for the three stations during dry season were 5.04±0.001a, 4.97±0.001b and
4.96±0.001b respectively, Station A was significantly different from Station B and C. While the total heterotrophic
bacterial counts during rainy season were 4.97±0.001a, 4.89±0.001b and 4.97±0.001a with Station B significantly
different from Station A and C. The total Pseudomonad counts (CFU/ml) showed significant difference amongst
the stations during dry season with 3.97±0.10a, 3.85±0.10b and 4.14±0.10c respectively also total pseudomonad
count (CFU/ml) during rainy season showed significant difference (P < 0.05) within the stations, 2.43±0.001a,
3.17±0.001b and 4.02±0.001c respectively. Similarly total coliform count ranged from 6MPN/100ml to
11MPN/100ml and 11MPN/100ml to 21MPN/100ml for dry and raining season respectively. Ten genera of
bacteria isolates were identified: Alcaligenes (3.3%), Bacillus (3.9%), Escherichia coli (15.6%), Klebsiella (14%),
Pseudomonas (12.7%), Salmonella (8.8%), Seratia (2.3%), Shigella (17.9%), Staphylococcus (4.2%) and Vibrio
(17.3%). The result of antimicrobial analysis revealed that of the ten antibiotic used the Pseudomonad isolates were
susceptible to Amoxacillin (25%,), Gentamycin(22.9) and Amoxacillin (22.9%). The isolates were highly resistant
to Augumentin (81.3%) and Streptomycin (64.6%). Isolate displayed site- difference in resistance and
susceptibility pattern. The need to monitor water quality and improve awareness of the risk to infections associated
with Pseudomonas aeruginosa is essential. Adherence to appropriate antibiotic use should be encouraged to
minimize the emergence of multidrug resistant bacteria.

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