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World Journal of Pharmaceutical
and Medical Research

An International Peer Reviewed Journal for Pharmaceutical and Medical Research and Technology
An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)
ISSN 2455-3301
IMPACT FACTOR: 4.639

ICV : 78.6

Abstract

COMPARISON BETWEEN MODIFIED ZIEHL NEELSEN AND SAFRANIN METHYLENE BLUE STAINING TECHNIQUES FOR DIAGNOSIS OF CRYPTOSPORIDIUM INFECTION AMONG DIARRHEIC PATIENTS IN RABAK CITY, WHITE NILE STATE, SUDAN

Ibrahim M. Hassan*, Abdalmoneim M. Magboul, Hafiz Y. Mohammed, Mohammed A. Suliman, Rabah M. Ibrahim, Ammar A. Abdalla, Afrah B. Hashim and Sara M. Abdalbagi

ABSTRACT

One hundred and twenty stool samples were collected from diarrheic patients at Rabak teaching hospital, white Nile state and examined to compare the results of Cryptosporidium infection obtained by Ziehl Neelsen and safranin methylene blue staining techniques, to determine the prevalence of the disease among those patients and to determine the other intestinal parasitic diseases in the study area. Samples were examined using direct saline, formal ether concentration and the two staining technique. Direct saline and formal ether concentration techniques give negative result for cryptosporidiosis, the prevalence were found to be 43(35.8%) and 38(31.7%) using modified Ziehl Neelsen and safranin methylene blue stain respectively. The study showed that the modified Ziehl Neelsen staining technique is most sensitive and accurate than safranin methylene blue staining technique. High prevalence of parasite was detected in male 25(58.1%) and 22(57.9%) than female, in age group one 32(74.4%) and 31(81.6%), in those with history of fever and weight loss 27(63%) and 25(65.8%) and in patient consuming pipeline water 39(90.7%) and 36(94.7%). Other intestinal parasites detected using direct and formal ether concentration techniques were, G. lamblia, E . histolytica, E . coli and H. nana. The study recommended to include the detection of Cryptosporidium oocysts in routine ova and parasite diagnosis using modified Ziehl Neelsen as first choice and safranin methylene blue stain as alternated technique and raise awareness of cryptosporidiosis in public health including personal hygiene and sanitation to limit its transmission.

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