Abstract

Sumangala Rao* and Navabharath

ABSTRACT

Objective: To isolate and screen potential antibiotic producing Actinomycetes from hospital dump yard soil and sediments of Bhadrakali reservoir, Warangal. Methods: Soil dilution – plating method was used to isolate Actinomycetes. Cross-streak method for primary screening, well-diffusion method for secondary screening of crude extracts and TLC to detect secondary metabolites was performed. Results: Of the twelve isolates of soil and freshwater sediments, eight were potential producers of antimicrobial metabolites. One soil isolate and three aquatic Actinomycetes were effective inhibitors of pathogenic bacteria employed. Conclusions: The present study is aimed to screen Actinomycetes from dump yards and to screen sediments as novel sources of antibiotics. The isolation of potential secondary metabolites producing Actinomycetes may help to meet the need of search of new antimicrobials. Most of the isolates from water sediments exhibited good antimicrobial activity, which can be further used in new drug discovery.