Abstract

Ranjit Kumar*, Pratibha Kumari, Shalini Kumari, Arun Kumar, Md. Ali and Ashok Ghosh

ABSTRACT

Arsenic found widely in the earth’s crust. It is mostly found in organic, inorganic and gas form. In some areas of the world, high levels of arsenic are naturally present in drinking water which leads to high toxicological concern. Arsenic commonly causes arsenicosis, melanosis, renal dysfunction and prolonged exposure may cause cancer of skin, bladder and liver. Probiotics are reported to have antimutagenic, anticarcinogenic, hypocholesterolemic, antihypertensive, anti-osteoporosis and immuno-modulatory effects. Lactobacillus sporogenes is common probiotics reside in intestine, it secretes a bacteriocin and coagulin, which is active against a broad spectrum of enteric microbes and causes detoxification. The present study is designed to evaluate hepatoprotective effect of Lactobacillus sporogenes against arsenic induced toxicity in Mus musculus. Mice were administered arsenic for four weeks followed by four weeks administration of Lactobacillus. Blood were collected for biochemical assay. Liver tissues were fixed for Light microscopic study. Arsenic causes many fold increase in SGPT and bilirubin level. Hepatic cells and kupffers cells were degenerated to greater extent with fragmented nuclei in arsenic exposed group. Sinusoid and central vein were dilated in arsenic exposed group. While lactobacillus causes marked restoration in biochemical and histological parameters of liver of mice. It is concluded from study that Lactobacillus causes effective restoration in SGPT and bilirubin level. Hepatic cells, kupffers cells, sinusoid and central vein were also restored effectively. It also maintains normal architecture of liver tissues. Lactobacillus reduces hepatotoxicity caused by arsenic effectively.