ISOLATION AND CHARACTERIZATION OF AZURIN PRODUCING PSEUDOMONAS SPP. AND OPTIMIZATION OF PRODUCTION PROCESS
Sabale S. S.*, Koli A.D., Marathe R. J. and Phatake Y. B.
The secondary metabolites from microorganisms play a vital role in developing new chemotherapeutics. Microorganisms especially bacteria produce different secondary metabolites which had proved to be one of the most important source of lead compounds. In the present study potent azurin producing bacteria were isolated and screen from from three soil samples (Maharashtra, India) and identified by morphological, biochemical and molecular method (16s r DNA) as Pseudomonas aeruginosa which produce a biologically active protein azurin, that has array of bioactivities. The produced azurin was extracted and qualitatively characterized by using conventional and modern analytical methods. The spectroscopic study shows lambda max of produced protein as 225nm. Finally the production process of azurin was optimized by classical method. The selected strain of bacteria produces maximum azurin in selective media-I containing yeast extract and potassium nitrate as carbon and nitrogen source respectively. The optimum pH for the yield of azurin was found to be 7. The time and temperature of incubation was also optimized as 24 hrs. and 37?C respectively. Optimum speed of agitation for the azurin production was found to be 150rpm.[Full Text Article]