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World Journal of Pharmaceutical
and Medical Research

An International Peer Reviewed Journal for Pharmaceutical and Medical Research and Technology
An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)
ISSN 2455-3301
IMPACT FACTOR: 4.103

ICV : 78.6

Abstract

METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF AMPICILLIN AND CLOXACILLIN CAPSULES BY USING RP-HPLC

Thushara B. S.*

ABSTRACT

A simple, Accurate, Precise and rapid reverse phase high performance liquid chromatographic isocratic method has been developed for the quantification of method AMPICILLIN and CLOXACILLIN Capsule (oral dosage form). The chormatograpic separation was attained on P.Hypersob C18 (250mm x 4.6, 5 ?m,)with precolumn as the stationary phase at ambient temperature .The Mobile phase was composed of Acetonitrile and 0.002 M phosphate buffer pH 3.0, 36:64 with a flow rate of 1.2 ml/min, and UV detection at 225 nm. The run time was 10 minutes. The injection volume was 20. The retention time of ampiciiln and cloxacillin were 1.32 minutes and 6.1 minutes respectively. The linearity in the Ampicillin drug concentration range of 0.1814 ?g/mL to 0.2236?g/mL and Cloxacillin the drug concentration range is 0.1718 ?g/mL to 0.2584 ?g/mL. The correlation coefficient were greater than 0.99. The mean percentage recovery of Ampicilln & Cloxacillin at 80%, 100%, and 120% concentration levels were for 105.43%, 103.78%, 101.36 & 105.81%, 105.27%, and 108.69% respectively. The results obtained show a method that is simple, rapid and precise. It can be used for the estimation of Ampicillin, Cloxacillin in single as well as combination pharmaceutical dosage forms.

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