STABILITY INDICATING RP-HPLC METHOD DEVELOPMENT AND VALIDATION FOR THE ESTIMATION OF ATAZANAVIR SULFATE IN BULK AND PHARMACEUTICAL DOSAGE FORM
Pavan Kumar V.*, P. Penchalamma, B. Sivagami, S. Charumathi, R. Sireesha and M. Niranjan Babu
ABSTRACT
The chromatographic conditions were successfully developed for the separation of Atazanavir sulfate by Agilent C18 Column (250mm x 25mm)5?m, flow rate was 1ml/min, mobile phase ratio was Methanol: Acetonitrile: Buffer (45:35:20 v/v), detection wavelength was 249 nm. The Spectroscopic method was done in solvent using mobile phase and the instrument Systronics 1170 with UV win software. The instrument used was Agilent HPLC, Separation module 2695, Uv-Vis detector, Empower-software version 2. The retention time was found to be 3.73 min. The system suitability parameters for Atazanavir sulfate such as theoretical plates and tailing factor were found to be 6754, 1.62. The analytical method was validated according to ICH guidelines (ICH, Q2 (R1))1. The linearity study of Atazanavir sulfate using internal standard was found in concentration range of 25 ?g-150 ?g and correlation coefficient (r2) was found to be 0.999 respectively, % recovery was found to be 100.5% respectively. % RSD for repeatability and precision was found to be
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