Abstract

M. Swapna, Asish Bhaumik*, K. Neelima Devi, G. Neelamma, Sreedevi

ABSTRACT

DU145 (DU-145) and PC3 human prostate cancer cell lines are the "classical" cell lines of prostatic cancer. DU145 cells have moderate metastatic potential compared to PC3 cells which have high metastatic potential. The DU145 cell line was derived from brain metastasis. DU145 are not hormone-sensitive and do not express prostate-specific antigen (PSA). It has been demonstrated that administration of NFkappaB ligand RANKL promoted DU145 cell invasion in bone, resulting in osteolytic lesions. DU145 cells also produce soluble factors that activate pre-osteoblast precursors and increase RANKL expression, thus facilitating prostate cancer metastasis in bone.The main objective of the present research work is the evaluation of in vitro cytotoxic activity of various extracts (METP, EETP and CETP) of turmeric powder (Curcuma longa). The in vitro cytotoxic activity was carried out against human prostate cancer cell line DU-145 by SRB assay. The results obtained from the in-vitro studies performed by SRB assay by using human prostate cancer cell line DU-145 displayed that the extracts METP, EETP and CETP possessed a very good cytotoxic activity. From the present studied it had been concluded that METP, EETP and CETP, all were exhibiting the potential cytotoxic action on DU-145 cell line which was proved by using standard drug 5-FU and it was found that METP, with the highest 92.80% cell growth inhibition at 10 ?g (IC50 = 2.5 ?g/ml), EETP with the 93.83% cell growth inhibition at 10 ?g (IC50 = 2.3 ?g/ml) and CETP with the 94.52% cell growth inhibition at 10 ?g (IC50 = 2.1 ?g/ml). The IC50 value of standard drug 5-FU was found to be 1.5 ?g/ml with 96.54 % growth inhibition at concentration 50 ?g/ml.