Abstract

Surbhi Chourasiya*, Dr. Versha Sharma and Dr. Anand Kar

ABSTRACT

The present study is focused on the elemental mapping and validation of extraction and characterization of Bacopa monnieri (Brahmi), the only used herb in Ayurveda; it shows the scavenging activities towards the free radicals, which are induced due to the exogenous chemical sources. We have mainly focused on the Bacopa monnieri plant herbal extraction of crude and characterization of its bioactive constituents like alkaloids, flavonoids, and bacosides. The BM herbal plant was collected from the botanical garden of Dr. Harisingh Gour Vishwavidyalaya Sagar and then identified by the Botany department via the herbarium file. Then, the dried plant was used for extraction by the Soxhlet method. The extract was dried in the oven, and the powder extract was used for the phytochemical screening and biological activity like Free radical scavenging activity or antioxidant activity, and H2O2 scavenging activity. We also perform SEM elemental mapping, FTIR, and NMR analysis. The results revealed that the extract possesses alkaloids, carbohydrates, terpenoids, Bacoside A, and Bacoside B as principal bioactive constituents. This study highlights the chemical constituents and biological properties of Bacopa monnieri. The study further validates its properties to promote it as a promising therapeutic effect.