Abstract

Surbhi Chourasiya*, Dr. Versha Sharma and Dr. Anand Kar

ABSTRACT

The present study is focused on the elemental mapping, validation and characterization of the extract of Bacopa monnieri (Brahmi), a commonly used herb in Ayurveda, that shows the scavenging activities towards the free radicals, which are induced due to the exogenous chemical sources. We have mainly focused on the Bacopa monnieri (BM) plant extraction and characterization of its bioactive constituents like alkaloids, flavonoids, and bacosides. The BM plant was collected from the botanical garden of Dr. Harisingh Gour Vishwavidyalaya, Sagar, and then identified by the Botany department from the herbarium file. The dried plants were used for extraction by the soxhlation method. The extract was dried in the oven, and the powder extract was used for phytochemical screening and biological activities such as free radical scavenging, antioxidant activity, and H2O2 scavenging activity. We also performed SEM elemental mapping, FTIR, and NMR analyses. The results revealed that the extract possesses alkaloids, carbohydrates, terpenoids, bacoside A, and bacoside B as principal bioactive constituents. This study highlights the chemical constituents and biological properties of Bacopa monnieri. The study further validates its properties to promote it as a promising therapeutic agent.