Abstract

Mamatha B. M.*, Ravindra Angadi, Radhika Ranjan Geethesh P., Ashok Kumar B. N. and Sushmitha V. S.

ABSTRACT

Introduction: Rasoushadhi, also known as Rasashastra, is an ancient branch of Ayurveda that deals with the preparation and use of herbal and mineral based medicines. The term a ‘Rasoushadhi’ is derived from two words. Rasa meaning essence or mercury, and Oushadhi meaning medicine. Rasoushadhi combines alchemical principles with ayurvedic philosophy to create potent, bioavailable medicines from natural substances. Tryambakaabhram is one of the herbo-mineral formulation which is having Abhraka as mineral drug along with herbal drugs as Bhavana Dravyas. It is mentioned in the Bhaishajya Ratnavali Swarabhedachikitsa. A detailed pharmaceutical and analytical study of Tryambakaabhram is essential to assess its safety, effectiveness, and acceptability. Therefore, certain physicochemical evaluations were conducted based on the parameters set by API and CCRAS. Aims & objectives: To prepare Tryambakaabhram as per the reference of Bhaishajya Ratnavali and to carry out detailed analytical study. Materials & Methods: The raw drugs are procured and subjected for further pharmaceutical process as per Bhaishajya Ratnavali. Observation & results: In pharmaceutical study the drug has been prepared by adopting Khalviya Rasayana method. The final drug prepared in our practical lab at the weight of 43gm. The final product of the drug was analysed with modern analytical methods viz. Physico-chemical standards, HPTLC, Pharmaceutical standards and Preliminary organic analysis. Discussion: Tryambakaabhram, prepared using the Kharaleeya Rasayana method, involved the Bhavana process with 11 Bhavana Dravyas, enhancing its therapeutic properties by reducing particle size and improving bioavailability. It was presented as a soft, dark brown powder with a characteristic odor and slightly bitter taste. Analytical studies revealed its moisture content (7.61%), total ash (68.92%), and higher water-soluble extractive value (25.71%), indicating good quality and bioavailability, with HPTLC analysis showing key Rf values at 254 nm, 366 nm, and post-derivatization at 620 nm. Conclusion: The Bhavana procedure in Tryambakaabhram transformed the phytoconstituents of Swarasa and Kashaya into Abhraka Bhasma, reducing particle size and improving absorption of therapeutic properties. Smaller particles enhance the absorption of active principles, improving efficacy. Analytical tests confirmed that Tryambakaabhram met quality standards, ensuring its intended use.