Abstract

Dr. Abdalmoneim M. Magboul*, Hafiz Y. Mohammed, Mohammed A. Suliman, Ibrahim M. Elhassan, Rabah M. Ibrahim, Ammar A. Abdalla, Alaa B. Abdelfatah and Neima M. Mohammed

ABSTRACT

Two hundred stool samples were collected from four basic school children in Kosti city, Kosti Province, White Nile State and examined to assess the use of poly vinyl alcohol (P.V.A) in smears prepared from Balamuth’s medium stool culture, to compare the results of different direct methods with culture method and to determine the prevalence of amoebic infection among those children. The samples were examined using normal saline method, formal ether concentration method, culture method without P.V.A and culture method with P.V.A. The total number of intestinal amoeba detected by normal saline method was 42 (21%), by formal- ether concentration method was 70 (35%), by culture method without P.V.A was 79 (39.5%) and by culture method with P.V.A was 86 (43%). The study showed that the culture method is more sensitive than the conventional methods (P>0.000). Stool culture when fixed with P.V.A was more reliable and effective in detecting trophozoites and cysts of intestinal amoeba than the same technique without P.V.A and the two are more efficient than wet preparation and formal ether concentration techniques.