Abstract

Akeem A. Agboke* and Odudu O. Etukudo

ABSTRACT

This study was designed to investigate the antibacterial and antifungal activity of the ethanol extract of Garcinia kola leaf. Microorganisms used in the study were Pathogenic, Staphylococcus aureus, Escherichia coli, Bacillus substilis, Pseudomonas aeruginosa and Candida albicans. The result of the sensitivity test showed that at a concentration of 100 mg/ml of the extract had a higher activity against Staphylococcus aureus with inhibition zone of 30mm and Bacillus substilis inhibition zone of 20mm, whereas no activity against Escherichia coli, Pseudomonas aeruginosa and Candida albicans and with no zone of inhibition. At a concentration of 50 mg/ml the crude extract showed activity against Staphylococcus aureus with inhibition zone of 20mm and Bacillus substilis with inhibition zone of 15mm. At a concentration of 25 mg/ml, the crude extract showed activity against Staphylococcus aureus with inhibition zone of 12mm and Bacillus substilis with inhibition zone of 8mm. At concentrations of 12.5 mg/ml and 6.25 mg/ml, there was no activity against any microorganism used in the study. The result of minimum inhibition testing showed the MIC of crude extract against Staphylococcus aureus and Bacillus substilis to be 25 mg/ml, while other microorganisms did not have MIC. The result of minimum Bactericidal Concentration (after 48hours) of the crude leaf extract showed the MBC of the crude extract against S. aureus 25mg/ml and 50 mg/ml against B. substilis. This research confirmed the antimicrobial activity of the leaf extract of Garcinia kola against some pathogenic gram positive bacteria more potent than Azithromycin at concentration of 1500mg/ml that had no effect on the S. aureus and B. Subtilis. It is also confirmed that the crude extract has no activity against gram negative bacteria and Candida Albicans.