Abstract

Asaad M. Mosawi*, Shaymaa Z. Nada and Hassan M. Abo Al-maali

ABSTRACT

Introduction: Type2 Diabetes mellitus (DM) was associated with progressively increasing morbidity and mortality. It is a complex multigenic disease with severe consequences. Single Nucleotide Polymorphism (SNP rs1861978 T>G) in dipeptidyl peptidase-4 (DPP-4) and Incretin hormones like Glucose-Dependent Insulinotropic Polypeptide (GIP) have been suggested to be associated in Type2Diabetes mellitus. Aim: To estimate the serum levels of Glucose-Dependent Insulinotropic Polypeptide (GIP) and study if there is an association between its levels and dipeptidyl peptidase-4 (DPP-4) gene polymorphism (rs1861978 T>G) in T2DM group and compare their levels with healthy control group and study their correlations with other biochemical parameters. Materials and Methods: The circulating GIP and DPP-4 gene polymorphism (rs1861978 T>G) were measured in 120 patients (63 male and 57 female), and 120 healthy control (65 male and 55female) without a known family history of diabetes with matched age (age range 44-55years) they were attending Al-Hassan diabetic and endocrinology care center in Imam Hussein Medical City / Kerbala - Iraq during the period from Jan. , 2018 to Nov. 2018. ELISA kit was used to measure S.GIP, DNA extraction kit and arms polymerase chain reaction (PCR) was used for DPP-4 gene polymorphism (rs1861978 T>G) Polymerase Chain Reaction (PCR) technique and the PCR product was visualized by agarose gel electrophoresis, and enzymatic methods were used for blood sugar, and lipid profile were measured for each subject. Results: The clinical characteristics showed a significant increase (pG) single nucleotide polymorphism has more role in the pathogenesis of T2DM than the T allele, and could be considered as a risk factor for T2DM in Iraqi population, as well as the association of the G/G genotype with the incidence of T2DM in Iraqi patients. DPP-4 (rs1861978 T>G) single nucleotide polymorphism was significantly associated with GIP level in T2DM group than control.