Abstract

D. Prasanthi*, Ch. M.M. Prasada Rao and D. Dhachinamoorthi

ABSTRACT

A simple, Accurate, precise method was developed for the simultaneous estimation of the Pregabalin and Epalrestat in Tablet dosage form. Chromatogram was run through Std Azilent 150 x 4.6 mm, 5?. Mobile phase containing Buffer: Acetonitrile taken in the ratio 45:55 was pumped through column at a flow rate of 1.0 ml/min. Buffer used in this method was 0.1% OPA buffer. Temperature was maintained at 30°C. Optimized wavelength selected was 241 nm. Retention time of Pregabalin and Epalrestatwere found to be 2.930 min and 2.179 min. %RSD of the Epalrestat and Pregabalin were and found to be 0.4and 0.2 respectively. % Recovery was obtained as 98.98% and 99.32% for Epalrestat and Pregabalin respectively. LOD, LOQ values obtained from regression equations of Epalrestat and Pregabalin were 0.02, 0.06 and 0.26, 0.77 respectively. Regression equation of Epalrestat is y = 20545x + 16173, and y = 18476x + 10803of Pregabalin. Retention times were decreased and run time was decreased, so the method developed was simple and economical that can be adopted in regular Quality control test in Industries.