Abstract

Gella Sasi Kala*, Gadi Vijaya Lakshmi, Yandamuri Narayudu and Kanchumarti Namratha

ABSTRACT

A simple, Accurate, precise method was developed for the simultaneous estimation of the Levodropropazine and Chloropheniramine Maleate in syrup dosage form. Chromatogram was run through Denali C18 150 x 4.6 mm, 5?. Mobile phase containing Buffer 0.02N KH2PO4 (3.47ph): Acetonitrile taken in the ratio 65:35 was pumped through column at a flow rate of 0.8 ml/min. Buffer used in this method was 0.02N KH2PO4. Temperature was maintained at 30°C. Optimized wavelength selected was 252 nm. Retention time of Levodropropazine and Chloropheniramine Maleate were found to be 2.300 min and 3.187 min. %RSD of the Levodropropazine and Chloropheniramine Maleate were and found to be 0.6 and 0.6 respectively. %Recovery was obtained as 100.12% and 100.40% for Levodropropazine and Chloropheniramine Maleate respectively. LOD, LOQ values obtained from regression equations of Levodropropazine and Chloropheniramine Maleate were 0.63, 1.90 and 0.09, 0.27 respectively. Regression equation of Levodropropazine is y = 16729x + 5154. And y = 52826x + 2622. of Chloropheniramine Maleate. Retention times were decreased and run time was decreased, so the method developed was simple and economical that can be adopted in regular Quality control test in Industries.