ANALYTICAL METHOD DEVELOPMENT AND VALIDATION OF NORFLOXACIN IN BULK AND DOSAGE FORMS USING UV SPECTROPHOTOMETRY
Y. Ratna Sindhu*, B. Ankamma Chowdary G. Mrudhula, S. Rajesh and K. Saisumanth
ABSTRACT
An attempt was made to develop simple and economical methods for the estimation of norfloxacin by UV method. In absorption 278 nm was selected wave length. The statistical analysis of data obtained for the calibration curve of norfloxacin in pure solution indicated a high level of precision for the proposed method, as evident by low relative standard deviation. The correlation coefficient was found to be significant. The linearity range showed straight line passing through origin. The method was validated by accuracy, precision and low values of % RSD results of recovery studies also proves the accuracy of method. For UV method methanol is selected as solvent which shows a maximum absorbance than other solvents like sodium hydroxide, HCL. The linearity was obtained for norfloxacin 4-16gm/ml. The precision was confirmed by low values of S.D and % RSD less than 2% conclude that no changes in validation parameter.
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