Abstract

Saja Talib Ahmed*, Salah Mahdi Al-Silaykhee, Zuhair Mohammed Ali Jeddoa and Mufeed J. Ewadh

ABSTRACT

Background: Diabetes mellitus is a metabolic disorder characterized by the presence of chronic hyperglycemia accompanied by greater or lesser impairment in the metabolism of carbohydrates, lipids and proteins. Diabetic foot ulcer (DFU) is complications of diabetes that affect the lower extremities are common, complex, and costly. The vascular endothelial growth factor is encoding VEGF that encompass 14 kb is located in chromosome region 6p21.3 and contains 8 exons and 7 introns. The current study deals with more frequent one (-2578 T/A) (rs699947). Objective: To investigate the molecular basis of VEGF (-2578 T/A) (rs699947) polymorphism and its correlation with biochemical parameters (HbA1c, GSH and MDA) in patients had DFU, in Kerbala province, Iraq. Materials and Methods: This study was a cross - sectional study. Sample size is 240 persons of both sexes randomly selected were conducted in the study divided into two groups, 120 had DFU and 120 had T2DM as control groups. Genotyping of VEGF was performed by amplification refractory mutation system Polymerase Chain Reaction (ARMS-PCR) of DNA extracted from peripheral blood mononuclear cells of 120 unrelated patients and 120 unrelated control donors. The most important SNP that affected on VEGF gene were involved in current study (-2578 T/A) (rs699947). Results: The significant results (p value ? 0.01) that showed in biochemical parameters (HbA1c, GSH and MDA) between DFU patients and T2DM as control group. The VEGF (-2578 T/A) (rs699947) polymorphism has a significant association with DFU. The AT genotype significantly raised the risk of DFU (p value ? 0.01). While the T allele significantly raised the risk of DFU (P value ? 0.01). The correlation of genotypes of VEGF (-2578 T/A) (rs699947) polymorphism with parameters (HbA1c, GSH and MDA) between DFU patients and T2DM as control groups, the correlation of AA genotype with parameters between DFU patients and T2DM as control group were significant (P value ? 0.01), but the correlation of AT genotype with parameters between DFU patients and T2DM as control group were significant (P value ? 0.01) while the correlation of TT genotype with parameters between DFU patients and T2DM as control group were significant (P value ? 0.01). Conclusion: The significant correlations were observed between (HbA1c, GSH and MDA) in DFU patients and T2DM as control group. The significant association between VEGF (-2578 T/A) in (rs699947) gene polymorphism and DFU patients as compared with T2DM as control group.