DEVELOPMENT OF VALIDATED ANALYTICAL METHOD FOR ESTIMATION OF IVABRADINE IN PURE AND PHARMACEUTICAL DOSAGE FORM USING RP-HPLC
Vuyyuri Kasi Viswanath* and Dr. Ch. M. M. Prasda Rao
ABSTRACT
A simple, Accurate, precise method was developed for the simultaneous estimation of the Ivabradine tablet dosage form. Chromatogram was run through Discovery C8 250mm x 4.6 mm, 5?m. Mobile phase containing KH2PO4: Acetonitrile taken in the ratio 50:50 was pumped through column at a flow rate of 1.0 ml/min. Temperature was maintained at 30°C. Optimized wavelength selected was 260.0nm. Retention time of Ivabradine was found to be 2.517min %RSD of the Ivabradine was found to be 0.6. % Recovery was obtained as 99.65% for Ivabradine, LOD, LOQ values obtained from regression equations of Ivabradine were 0.31, 0.95 and 0.68,Regression equation of Ivabradine is y = 17301x + 1713. Retention time, runtime was reduced, so the technique created has been easy and economical that can be used in periodic quality control tests in industries.
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