Abstract

Sanhouri Kanan*, A.A.Mohamadani, A.M. ELHAJ, Ihsan. M. Osman, Abbas A.A

ABSTRACT

Objective: Proper Characterization of lymphoma cases attending the medical lab by using immunohistochemistry Material and Methods: Fourty cases already diagnosed as lymphoma in the period Jan 2007 to Dec 2008 at Medical laboratory University of Gezira, Sudan. The cases were selected from the records and accordingly the paraffin embedded blocks were collected. Re-embedding of the blocks were done. From these blocks the haematoxylin and eosin stain was performed to assess the preservation of tissue as well as to select the suitable blocks to perform the immunohistochemistry on it. Results: show similar male/female ratio for Hodgkin's and non-Hodgkin's lymphoma .In Hodgkin's lymphoma the cells showed positivity for CD3, CD20, CD15 and CD30 with percentages of 87.6%,85.7%,78.6% and 57.1% respectively. The atypical cells in case of non-Hodgkin's lymphoma showed positivity for CD45 with a percentage of 100%, CD3 &CD20 in 23 cases representing 88.5% for each. Twelve cases were found to be CD15 positive with a percentage of 46.2 %, while the remainder 5 cases representing 19.2 % were found to be CD30 positive. Conclusion: The study concluded that the use of immunohistochemistry is very important for diagnosis of lymphoma beside H and E to avoid diagnostic pitfalls. Interpretation of marker studies must be based on a panel and knowledge of a particular antigen's expression in normal, reactive, and neoplastic conditions.