Abstract

Blashkiv Taras*, Sribna Valentyna, Kutsevol Natalia, Kaleinikova Oksana, Kuziv Yulia, Voznesenskaya Tetiana

ABSTRACT

Recently, it has been proved that polymers with a dextran core and grafted polyacrylamide chains (D-g-PAA) were effective nanoplatform for creation of nanocomposites for chemotherapy and photodynamic therapy. Also, these polymers can be like a matrix for stable silver sol synthesis, that gives confidence in the prospect of silver-based nanosystems for biomedical application. An animal studies are becoming more relevant to determine the effect of the treatment of nanosilver on female reproductive function in particular. In this work for the first time we have obtained data about the influence of star-like D70-g-PAA polymers on somatic, germenative cells, embryos and live newborns (pups) in mammals, which had not previously been investigated. No significant changes in meiotic maturation of such ovarian oocytes in vitro, the number of living cells of FEO and the number of such cells with morphological signs of apoptosis and necrosis, pre- and post-implantation mortality rates of embryos and the number of live newborns (pups) have been established under conditions of one-time treatment with D70g-PAA and D70-g-PAA(PE) at doses of 0,39 mg/kg and 3,90 mg/kg and AgNPs/D-g-PAA and AgNPs/D-g-PAA(PE) at doses of 0,20 mg/kg and 2,00 mg/kg. The effect of AgNPs/D-g-PAA(PE) at a dose of 2,00 mg/kg on oocytes is inhibitory (reduces the number of oocytes in the ovary). The inhibitory effect of such polymer D70-g-PAA (PE) at a dose of 3,90 mg/kg on oocytes was also established. However, there is no additional effect of AgNPs/D70-g-PAA(PE) on oocytes compared to the effect of the polymer itself (D70-g-PAA(PE). The inhibitory effect of AgNPs/D70-g-PAA and AgNPs/D70-g-PAA(PE) on cells of ILN has been established. However, the effect of D70-g-PAA and D70-g-PAA(PE) on cells of ILN is not suppressive. An additional effect of AgNPs/D70-g-PAA(PE) (2.00 mg/kg) on cells of ILN was also compared with the effect of D70-g-PAA(PE) polymer itself.