DEVELOPMENT AND VALIDATION OF NEW UV SPECTROPHOTOMETRIC METHODS FOR THE ESTIMATION OF BENFOTIAMINE IN BULK AND SOLID DOSAGE FORM
Niraimathi. V* and Siva Sankari K.A.
ABSTRACT
Benfotiamine (synonym S-benzoylthiamine-O-monophosphate) is the precursor of thiamine which is a water soluble vitamin. Benfotiamine is fat soluble, a characteristic that allows it to enter cells more readily than thiamine and thus helps to prevent diabetes-related dysfunction within the cells. This enhanced bioavailability makes benfotiamine particularly effective in treating hyperglycemia related damage to tissues and organs. Two simple, precise, accurate UVspectrophotometric methods namely dual wavelength spectrophotometric method and difference UV spectrophotometric method have been carried out using 0.1 N HCl as solvent. The drug obeyed Beer’s law in the range between 4-20?g/mL. The overall percentage recovery was found to be 99-101% which indicates the accuracy of the proposed method. The methods have been validated as per ICH guidelines and could be applied in future analysis of Benfotiamine formulations.
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