Abstract

A. Manoj*, B. Vishnu Bhat, C. Venkatesh and Z. Bobby

ABSTRACT

Lipid peroxidation is the oxidative degradation of lipids. It is the process in which free radicals steal electrons from the lipids in cell membranes leading to cell damage. The end products of lipid peroxidation are reactive aldehydes, such as Malondialdehyde (MDA). Malondialdehyde (MDA) is the organic compound colourless liquid which is a highly reactive compound that occurs as the enol. The production of this aldehyde is used as a biomarker to measure the level of oxidative stress. Malondialdehyde reacts with bases adenosine and guanosine in DNA, forming DNA adducts hence it well correlates with DNA damage and chromosomal aberrations. Malondialdehyde and other thiobarbituric reactive substances (TBARS) condense with two equivalents of thiobarbituric acid to give a fluorescent red derivative that can be assayed spectrophotometrically. The methodology for estimation of MDA was standardised for determining the oxidative stress induced DNA Damage in Perinatal Asphyxia. Peripheral veins samples were collected in heparinised tubes. Plasma were separated and stored in deep freezer and estimated the amount of MDA in oxidative stress DNA damages of cases and controls. MDA estimation is recommended for evaluation of oxidative stress induced DNA damage. There was significant elevation of serum MDA level in Perinatal asphyxia.