Abstract

*Dr. Baydaa Ali Othman Al-Rawi, Shahad Z. Al-Janabi and Nadia A. Abdulsahib

ABSTRACT

Aim of the Study: The aim of this study was to evaluate the potential role of Platelet Rich Plasma (PRP) in the stem cells from the apical papilla through the histopathological changes after using PRP to aid regeneration of non-vital immature teeth. Materials and Methods: Twenty local breed dogs of (4-6) months-old in good general health were used (approximately 6-9 kg body weight) in this study. The animals were anesthetized with a mixture of Xylazine 1mg/Kg body weight and Ketamine 5mg/kg. After infection and disinfection of teeth using triple antibiotic paste, PRP was introduced in treating teeth. Histopathological analyses carried out after 2, 4, 8 and 12 weeks. Results: At different time intervals, all teeth of Group (1) (antibiotic group) failed to demonstrate any increase thickening of canal walls, continue root development, apical closure or pulp like tissue in the root canal, meanwhile, there were differences in the images at different time intervals among treated teeth of Group (2). Appropriate outcomes of this treatment determined by the histopathological evaluations of this study which revealed that immature teeth with necrotic pulp and apical periodontitis after revascularization procedure of using PRP could increased thickening of canal walls, continued root development, apical closure with pulp like tissue in the root canal space in teeth of Group (2). Conclusions: Apical maturation of treating teeth continued in much the same fashion as in the untreated control teeth. PRP has increased concentrations of growth factors that can attract stem cells present in the apical tissues, aid in revascularization and stimulation of undifferentiated mesenchymal cells leading to the deposition of a calcific material at the apex as well as on the lateral dentinal walls of the canal.