Abstract

Ibrahim A. El Desoky, Eman M. Saleh, Atef S. Darwish and Rasha E. Hassan*

ABSTRACT

Cyclophosphamide (CP) is an effective anti-cancer alkylating agent, while it also possesses a wide spectrum of cytotoxicity as nephrotoxicity. Nanoparticles are used as drug carriers and imaging to increase drug solubility, stability and control drug release and specific transport and reduce drug toxicity. The current study was constructed to evaluate the ameliorating effect of the newly synthesized CP& Egg albumin /carbon nanotube nanocomposite (CP&EA/ CNT) against CP induced nephrotoxicity in rats and to investigate the effectiveness of the newly synthesized composite in treating nephrotoxicity. The physicochemical properties of the newly synthesized composite were also assessed by Characterization techniques such as TEM, SEM, zeta-potential, FT-IR and XRD. In vitro study (Cell viability assay) wherever the cytotoxic effect (IC50) of newly synthesized composite (CP&EA/CNT). To achieve this goal In vivo assay, 24 adult male rats were randomly divided into three equal groups (8 rats/group), (1) control group, (2) CP group, (3) CP&EA/CNT group. After experimental period, serum renal biomarkers (creatinine, urea, uric acid, BTP, nepherin, interleukin 8(IL8) and NGAL were evaluated. In addition, histopathological examination of kidney and was performed. Our results prove that CP caused nephrotoxicity shows increase in (creatinine, urea, uric acid, BTP, nephrin, IL8 and NGAL) levels for groups that were injected with CP only in healthy rats. Groups were injected with CP&EA/CNT nanocomposite revealed a significant improvement in renal biomarkers levels and also improve in histopathological disorders of kidney. Ultimately, the outcomes of this study ascertained the ameliorating role of the newly synthesized CP&EA/CNT nanocomposite against nephrotoxicity induced by CP in rats without interfering with CP anticancer properties.