Abstract

Rajesh Kumar A.* and Vennila G.

ABSTRACT

The generation of Reactive Oxygen Species (ROS) occurs constantly during normal cell metabolism in all living cells (Yu, 1994). Free radical generation that exceeds the capacity of antioxidant defences results in oxidative stress, which possibly elicits irreversible, degenerative response including apoptosis or necrosis, in living cells (Buttke and Sandstorm, 1994). Higher amounts of ROS play a role in the ageing process as well as in a number of human disease states, including cancer, ischemia, and failures in immunity and endocrine functions. As a safeguard against the accumulation of ROS, several non-enzymatic and enzymatic antioxidant activities exist. The purpose of this study is to determine whether inducible nitric oxide synthase (iNOS) is involved in the pathogenesis of testicular ischemia-reperfusion (I/R) injury in association with germ cell death, through either necrosis or apoptosis. The objective of our study was to investigate involving excessive iNOS expression shows intracellular ROS (NO) generation, during ischemia reperfusion injury, is identified by Immunohistochemistry, on both sides of testis (ipsilateral and contralateral) and antioxidant property of guava leaf extract may provide protection against testicular torsion ischemia reperfusion injury in rats.