Abstract

Neelofar Khanam*, Mohit Kumar Gupta and G. K. Sharma

ABSTRACT

Traditional plant-based remedies are finding increasing application as a source of direct therapeutic agent. Presently, plants are the original source materials for as many as 40% of pharmaceuticals currently used around the world. These drugs either contain plant-derived materials, or materials synthesized from agents originally derived from plants. Innumerable therapeutic agents can be obtained from medicinal plants after screening of secondary metabolite compounds. Since ancient time Aloe vera L. are using in many herbal preparations as antioxidants. Aloe vera L. possesses an important role in absorbing and neutralizing oxidant induced free radicals. In present investigation the enzymatic antioxidant activities of in vivo grown and in vitro propagated Aloe vera L. whole leaves and only gel extracts against oxidative stress induced in vitro animal model were examined. Here in current study, we used in vitro animal models as an alternate of using live animals in laboratory to reduce the harm and pain to live animals during laboratory experiments. Results were showing that among the different samples of methanol extracts of in vivo and in vitro regenerated Aloe vera L. whole leaves and inner gel, the methanol extracts with in vitro regenerated Aloe vera L. leaves were showing higher degree of protection against oxidant induced stress in in vitro animal model.