Abstract

K. K. Igwe*, N. K. Achi, Chika Ikenga, A. J. Madubuike, N. S. Nwatu, C. J. Onyenze, I. E. Otuokere, I. I. Ijeh and P. E. Aba

ABSTRACT

Anti-anaemia effect, GCMS analysis and molecular docking study of ethanol extract of Manihot esculenta leaves was studied on Phenylhydrazine induced anaemia using Wistar rats as animal model. Twenty five (25) rats were used for the research, they were grouped into five (5) of five (5) rats each. Group 1 was the negative control group and received distilled water. Groups 2 was the anaemic non treated group, 3, 4 and 5 were the treatment groups received 250, 500 and 1000 mg/kg body weight of the M. esculenta extract respectively. The rats were dosed for 14 days, thereafter were sacrificed and blood collected by cardiac puncture for analysis. The effect of M. esculenta, extract was checked on haematological parameters and serum enzymes activities. All results in treatment groups were compared with the untreated anaemic group at statistical confidence of 95% (p<0.05). The normal control group saved as reference point. The result shows that M.esculenta restored the blood cells in a dose dependent manner compared with those untreated anaemic group. The leukogram indicated lymphopenia, neutrophilia, monocytosis and eosinophilia. AST, ALP and ALP were within normal reference range indicating liver safty while bilirubin, urea and creatinine showed no kidney problem. Total protein was recovered in dose dependant manner compared with untreated anaemic group. Gas chromatogram showed 34 indicating 34 phytocompounds present in M.esculenta and the three most abundant compounds were Oleic acid, Squalene and 2-Methyl-Z,Z-3,13- octadecadienol. The lead compounds had good absorption, distribution metabolism and excretion with no toxicity and can be good candidate for drug production.