ISOLATION AND QUANTIFICATION OF STIGMASTEROL FROM METHANOLIC EXTRACT OF LEAVES OF COSTUS SPECIOSUS BY HIGH PERFORMANCE THIN LAYER CHROMATOGRAPHY
Apurva A. Tayade* and Kirti S. Laddha
ABSTRACT
The present study focusses on isolation and quantification of stigmasterol in methanolic extract Costus speciosus leaves. The method of isolation was simple involving extraction with methanol and further purification by column chromatography. The identity of stigmasterol was confirmed by infrared spectroscopy (IR) and nuclear magnetic resonance (NMR). A specific and rapid high performance thin layer chromatography (HPTLC) method was developed for analysis of stigmasterol for which separation was conducted on silica gel 60 F254 plates using n-Hexane: Ethyl acetate: Formic acid (8:2:0.1) as the solvent system. The method was observed to be linear in the range of 0.2-0.6 μg/ spot with correlation coefficient r2 = 0.996. The LOD and LOQ obtained were 37.8 ng and 114.56 ng respectively. Thus, the proposed validated HPTLC method is new and was found to be accurate and convenient and could be used for rapid screening and quality control of herbal formulations consisting of Costus speciosus.
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