ASSESSMENT OF LONG NON-CODING RNA LNC-RP11-697E AND ITS TARGET Hepatocyte Nuclear Factor 1 AS PREDICTORS TO CISPLATIN- RESISTANCE IN OVARIAN CANCER
Noha Ahmed Abdel Rahman, Nashwa El-Khazragy, Mohamed M. Naguib and Marwa G. A. Hegazy*
ABSTRACT
The current study aimed to investigate the prediction utility of the long noncoding RNA (lnc-RP11_697E) and its target gene, hepatocyte nuclear factor 1β (HNF-1β) to Cisplatin-resistance in patients with ovarian cancer (OC), in addition, to assess their diagnostic significance. The expression levels of lnc-RP11-697E and HNF-1β were assessed in 25 formalin-fixed paraffin-embedded tissue (FFET) samples from OC patients who were treated with intravenous Cisplatin-based chemotherapy and 25 healthy ovarian (non-cancerous) tissues as the control group using qPCR. The results revealed that lnc-RP11-697E was significantly overexpressed, while HNF-1β was downregulated in Cisplatin-resistant tissues. In addition, lnc-RP11-697E and its target HNF-1β exhibited high sensitivity and specificity in predicting Cisplatin-resistance and in differentiating ovary cancer from controls. The tissue expression levels of lnc-RP11-697E and its target HNF-1β could be used as diagnostic biomarkers for OC and as predictors for Cisplatin-resistance in patients suffering from OC.
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