Abstract

Ika Maharani, Athika Darumas Putri and Lia Kusmita*

ABSTRACT

Background: These guidelines Indonesia is a country with diverse biological resources, typically from marine resources, Spirulina platensis. The secondary metabolite compounds contained in Spirulina platensis are chlorophyll, phycocyanin, beta carotene, folic acid, phenolics, catechin hydrate and epicatechin, where these compounds have anti-obesity activity. One important factor in obtaining an extract with antioxidant and antiobesity activity is solvent optimization using an extraction method using fast maceration. This study aims to determine the effect of different extraction solvents to extract pigment compounds contained in Spirulina platensis on antioxidant and antiobesity activity with pancreatic lipase enzyme inhibitors in vitro. Methods: Spirulina platensis dry powder was extracted using the fast maceration method, using 4 solvents, namely 100% methanol, 100% acetone, methanol : acetone (70 : 30), acetone : methanol (70 : 30). Antioxidant activity test using DPPH and obesity activity with pancreatic lipase enzyme was employed using ELISA multimode reader. Result: The yields obtained from the maceration method from 100% methanol, 100% acetone, methanol : acetone (70:30), and methanol : acetone (30 : 70) were 13.10 ± 0.19%, 3.33 ± 0.46, 13.92 ± 0.78%, 9.26 ± 0.17 % respectively. The best antioxidant activity was found in the extract with methanol : acetone 30:70 solvent (79.84±0.62 µg/mL). The best lipase inhibitory activity was found in the extract with methanol : acetone 30:70 solvent (75.34±0.94 %).