THE MECHANISM OF FLUORESCENCE IN SITU HYBRIDISATION TECHNIQUE
Manoj A.* and Sourav R. M.
ABSTRACT
The Fluorescence in situ Hybridisation (FISH) is a molecular cytogenetic method to detect the presence or absence of specific genes in chromosomes by using radioactive fluorescent DNA dye/ Probes is for binding/hybridizing the exact locations of fixed DNA in which the dye emits bright spots during analysis under fluorescence microscope to see the area of interest. There are different formats of FISH method to investigate the area of interest. The protocol consists of Nine steps such as Preparation of slides, Pretreatment of slides, Probe preparation and additions, Denaturation, Hybridization, Post-hybridization wash, Counter staining, Visualization, Image capture and Analysis.
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