Abstract

Dr. Rajaputana Lakshmi Manisha*, M. Sri Sai Divya, Bachu Bhavani and Dr. Muvvala Sudhakar

ABSTRACT

Aim: Amaranthus roxburghianus Nevski Hydroalcoholic aerial plant extract was taken to treat Gentamicin induced nephrotoxicity in Wistar rats. Methodology: 30 male wistar rats were taken and randomly divided into 5 groups containing 6 animals. Group-I was considered as control treated with normal saline (10ml/kg p.o), Group-II was treated with Gentamicin (100mg/kg i.p), Group-III was treated with Gentamicin (100mg/kg i.p) and Vitamin-E (250mg/kg p.o), Group-IV and Group-V were treated with Gentamicin (100mg/kg i.p) and Hydroalcoholic Extract of Amaranthus roxburghianus Nevski (200mg/kg p.o and 400mg/kg p.o respectively) for 7 days. At the end of the treatment i.e. on 8th day the animals were sacrificed and biochemical changes in creatinine, urea, uric acid and total protein were estimated in blood. Histopathological evaluation of kidney was done. The statistical analysis was done. Results: The plant extract revealed Alkaloids, Flavonoids, Phenolic compounds, Tannins, Glycosides, Amino acids, Terpenoids, Steroids, Lipids, Saponins. In the treatment groups, the hydroalcoholic extract of plant had significantly reduced the increased levels of serum creatinine, urea, and uric acid levels and improved serum total protein levels. It also normalized the changes in the anti-oxidant parameters. Histological examination of the kidneys revealed that treatment with the extracts restored normal kidney structures in the animals treated with HARE 200mg/kg & 400mg/kg along with GEN as compared to GEN treated group.