Abstract

Ekpiken Solomon Ek piken* and Nneka Ndifon Agbiji

ABSTRACT

A. cordifolia has various pharmacological as well as therapeutic potentials. This present study was to determine antibacterial and phyto chemical potentials of A. cordifolia fractions against uropathogens and molecular docking of bioactive compounds. Standard procedures were used in crude extraction and fractionation of the extract. Antibacterial susceptibility was performed using standard protocol. Spectroscopic analysis as well as molecular docking of the bioactive compounds were carried out following standard protocol; and 16S rRNA sequencing standard protocol was used to confirm the uropathogens previously isolated from urine. Antibacter ial activities of the fractions revealed remarkable inhibitory activities against all the test bacterial isolates and were in the descending order: butanol>ethyl acetate>dichloromethane>aqueous. The MIC index showed that butanol fraction is bactericidal. G as Chromatography Mass Spectroscopy results revealed the presence of fifteen (15) and thirteen (13) compounds for butanol and ethyl acetate fractions respectively. Major phytoconstituents identified include: eicosanoic acid, 4H cyclopentacyclooctane, Ethyl iso allocholate and Pregna 5,16 dien 20 one, 3 --( 16 methyl --, ( from butanol fraction and Pregna 5,16 dien 20 one, 3 --( 16 methyl --, ( 3β)--, hexadecenoic acid, and 10 hydroxy, methyl ester from ethyl acetate. The drug likeness and phar macokinetic ADMET of the compounds indicated possible novel drugs for treatment of bacterial infections. These resul ts are promising and point to the possible use of Pregna 5,16 dien 20 one, 3 --( 16 methyl --, (3β) and Ethyl iso allocholate as alternative sources of antibacterial agent.