Abstract

P. Shivakumar Singh and G.M. Vidyasagar*

ABSTRACT

The main aim and object to calculate in-vitro antidermatophytic activity of methanolic young leaves extracts of Bixa orellana, this is the first report on antidermatophytic assessment. The antidermatophytic activity of high-polar methanolic young leaves extracts of Bixa orellana was evaluated by agar well diffusion scheme, the minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC), minimum bactericidal concentration (MBC) were determined using broth dilution method. The preliminary phytochemical tests for secondary metabolites were carried out in both the extracts. The antidermatophytic examination through the five fungal and five bacterial strains were experimented to determine the effective concentration from methanolic young leaves extracts of Bixa orellana. The high-polar methanolic extract was exhibited an efficient antidermatophytic activity. The maximum antidermatophytic activity was observed against C. albicans (20.00±0.00mm) followed by T. rubrum (12.66±1.15mm), M. gypseum (10.33±1.15mm), T. tonsurans (09.33±0.57mm) and T. Mentagrophytes (09.00±0.00mm). Whereas, the maximum antibacterial activity of 20.00±0.00 mm inhibition was observed in E. coli followed by B. subtilis (18.66±0.57mm), P. aeruginosa (17.33±0.57mm) and S. aureus (16.00±00mm). The zone of inhibition was found to be concentration dependent relative. The preliminary phytochemical tests for the detection of secondary metabolites were performed. The present first report provides a foundation for the isolation and purification of anti-dermatophytic molecule(s) from the young leaves methanolic extracts of Bixa orellana.