PRODUCTION, OPTIMIZATION AND APPLICATION OF TYROSINASE FROM BACILLUS SP CGR6 IN DYE DEGRADATION
Pranita Pradhan and Payel Sarkar*
ABSTRACT
The aim of the present study is to isolate a bacterial strain from dye contaminated soil. Bacterial strain CgR6 was selected as the best dye degrading isolate. Biochemical characterization concluded that CgR6 strain belongs to the genus Bacillus sp. After optimising the conditions in minimal media it was found that tyrosinase productionwas maximum in presence of glucose (1.09 EU/ml) and sodium nitrate (0.92 EU/ml). Addition of NaCl enhanced tyrosinase production (0.59 EU/ml. Sodium azide was found to be a potent inhibitor of tyrosinase enzyme causing reduction in tyrosinase production. Maximum tyrosinase activity was seen at dye concentration of 0.01% (1.2 EU/ml). the optimum temperature for tyrosinase was 50 ºC at pH 10. The enzyme was stable at a wide range of temperature (10ºC - 80 ºC) and a pH range of 8-11. Tyrosinase produced from strain CgR6 also had the ability to decolourization a wide range of dyes such as Congo red, Methyl red, Malachite green and Crystal violet. Highest percentage of decolourization was seen in Malachite green (91.9%). Thus, the tyrosinase produced by Bacillus CgR6 strain has immense potential in removal of dye wastes.
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